Review





Similar Products

chemiluminescence kit pierce ecl western blotting substrate  (Thermo Fisher)
90
Thermo Fisher chemiluminescence kit pierce ecl western blotting substrate
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Chemiluminescence Kit Pierce Ecl Western Blotting Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chemiluminescence kit pierce ecl western blotting substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
chemiluminescence kit pierce ecl western blotting substrate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
hrp-conjugated secondary antibody pierce ecl western blotting substrate  (Thermo Fisher)
90
Thermo Fisher hrp-conjugated secondary antibody pierce ecl western blotting substrate
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Hrp Conjugated Secondary Antibody Pierce Ecl Western Blotting Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp-conjugated secondary antibody pierce ecl western blotting substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
hrp-conjugated secondary antibody pierce ecl western blotting substrate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pierce® ecl western blotting substrate  (Thermo Fisher)
90
Thermo Fisher pierce® ecl western blotting substrate
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Pierce® Ecl Western Blotting Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pierce® ecl western blotting substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
pierce® ecl western blotting substrate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pierce® ecl plus western blotting substrate  (Thermo Fisher)
90
Thermo Fisher pierce® ecl plus western blotting substrate
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Pierce® Ecl Plus Western Blotting Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pierce® ecl plus western blotting substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
pierce® ecl plus western blotting substrate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
enhanced chemiluminescence kit pierce ecl western blotting substrate  (Thermo Fisher)
90
Thermo Fisher enhanced chemiluminescence kit pierce ecl western blotting substrate
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Enhanced Chemiluminescence Kit Pierce Ecl Western Blotting Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enhanced chemiluminescence kit pierce ecl western blotting substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
enhanced chemiluminescence kit pierce ecl western blotting substrate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
chemiluminescent pierce tm ecl-western blotting substrate  (Thermo Fisher)
90
Thermo Fisher chemiluminescent pierce tm ecl-western blotting substrate
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Chemiluminescent Pierce Tm Ecl Western Blotting Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chemiluminescent pierce tm ecl-western blotting substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
chemiluminescent pierce tm ecl-western blotting substrate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ecl western blotting substrate (pierce, cat #32106)  (Thermo Fisher)
90
Thermo Fisher ecl western blotting substrate (pierce, cat #32106)
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Ecl Western Blotting Substrate (Pierce, Cat #32106), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecl western blotting substrate (pierce, cat #32106)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
ecl western blotting substrate (pierce, cat #32106) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
hrp secondaries pierce ecl western blotting substrate  (Thermo Fisher)
90
Thermo Fisher hrp secondaries pierce ecl western blotting substrate
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Hrp Secondaries Pierce Ecl Western Blotting Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp secondaries pierce ecl western blotting substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
hrp secondaries pierce ecl western blotting substrate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pierce ecl western blotting substrate p0018a  (Beyotime)
90
Beyotime pierce ecl western blotting substrate p0018a
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Pierce Ecl Western Blotting Substrate P0018a, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pierce ecl western blotting substrate p0018a/product/Beyotime
Average 90 stars, based on 1 article reviews
pierce ecl western blotting substrate p0018a - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
electrochemical luminescence (ecl) substrate pierce ecl plus western blotting substrate  (Thermo Fisher)
90
Thermo Fisher electrochemical luminescence (ecl) substrate pierce ecl plus western blotting substrate
A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a <t>chemiluminescence</t> detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.
Electrochemical Luminescence (Ecl) Substrate Pierce Ecl Plus Western Blotting Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electrochemical luminescence (ecl) substrate pierce ecl plus western blotting substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
electrochemical luminescence (ecl) substrate pierce ecl plus western blotting substrate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a chemiluminescence detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.

Journal: Scientific Reports

Article Title: A conserved motif within the NSP2 of SARS-CoV-2 is required for processing of the distal NSP1/NSP2 junction by NSP3

doi: 10.1038/s41598-025-10244-2

Figure Lengend Snippet: A short, conserved motif within SARS-CoV-2 NSP2 is required for processing of the NSP1/NSP2 junction by the PL pro (NSP3). Cell lysates from BHK cells infected with vTF7-3 and transfected with plasmids that express the SARS-CoV-2 NSP1-NSP2-Flag (wt or with a small deletion, removing the conserved motif) either alone (odd numbered lanes) or with the plasmid encoding the SARS-CoV-2 PL pro (even numbered lanes) were analyzed by immunoblotting using rabbit anti-Flag antibodies, followed by anti-rabbit HRP-conjugated secondary antibodies and a chemiluminescence detection kit. Molecular mass markers (kDa) are indicated on the left. A negative control (no DNA) was included (lane 5). The uncut membrane is shown in Supplementary Fig. S1. This experiment has been repeated three times in total with separate transfections and western blots and resulted in very similar results. The additional two experiments are shown in Supplementary Figs. S2 and S3. Band intensities for each experiment were quantified using pixel count in ImageJ. Each lane was set to 100%, and the relative percentage of each of the two bands was calculated. The mean values of the triplicates were plotted, and the bar diagram with error bars representing the standard deviation (SD) are shown on the right side of the figure.

Article Snippet: Following these incubations, a chemiluminescence kit (Pierce ® ECL Western Blotting Substrate, Thermo Fisher Scientific) was used to detect the target proteins.

Techniques: Infection, Transfection, Plasmid Preparation, Western Blot, Negative Control, Membrane, Standard Deviation